Automating the T-SPOT.TB test
In TB testing, why trade accuracy for efficiency when you don’t have to?
The T-SPOT.TB test involves multiple steps which help to ensure accuracy when reporting latent tuberculosis (TB) results; but it no longer has to be a fully manual procedure. By choosing to automate just one, a number, or all of the processes within the workflow, your lab can select the most cost-effective solution for your specific needs and offer this clinically superior IGRA.1,2
What makes each workflow step important?
Cell Isolation | Enriches the desired population of peripheral blood mononuclear cells (PBMC) from whole blood collected using routine phlebotomy |
Cell Counting | Facilitates correction for variation in PBMC counts and ensures the required number of cells are used to produce a reportable result |
Liquid Handling | Essential for the transfer of samples and reagent solutions throughout the workflow for accurate test results |
Plate Development | Interferon gamma antibodies capture a cell specific response to TB-specific antigens |
Spot Counting | Single cell resolution for the detection of interferon gamma release |
The isolation of PBMCs from whole blood is critical
What is T-Cell Select™?
The cell isolation step of the T-SPOT.TB workflow is now automated using the T-Cell Select reagent kit eliminating manual errors and reducing technician time. This PBMC isolation reagent uses positive selection of targeted immune cells with magnetic bead-based cell separation to simplify the preparation of cells for the T-SPOT.TB test.
The T-SPOT.TB test with the T-Cell Select reagent kit provides you with:
- Superior clinical performance 1,2
- Reduced hands-on time
- Extended blood sample stability
- Improved blood sample logistics
- Maximized operational efficiency
- Increased sample throughput potential
FDA Approved Cell Isolation Platforms
Auto-Pure 20B | Auto-Pure 24 | KingFisher™ Flex | |
Throughput | Flexible | Medium-High | Medium-High |
Samples per run | Up to 20 | Up to 24 | Up to 24 |
Consumables | 5 mL sample tube strips + tip combs | 24 well, deep well plates + tip combs | 24 well, deep well plates + tip combs |
Run time | < 45 minutes | < 45 minutes | < 45 minutes |
Dimensions (W x D x H) | 400 x 520 x 450 mm | 560 x 620 x 500 mm | 680 x 600 x 380 mm |
Bench weight | 30 kg (66 lbs) | 54 kg (119 lbs) | 28 kg (62 lbs) |
Product flyer | [Download] | [Download] | [Download] |
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Take control with improved blood sample stability and logistics.
Samples stored at room temperature for 54 hours
Enables easy centralization of sample processing and further simplifies sample logistics.
- Flexibility for sample batching
- Simplify sample delivery
- Extend sample delivery
- Expand the reach of your laboratory
Simple sample logistics using routine phlebotomy
Ensure all up-front processing of the specimen after blood draw is done in a laboratory setting where controlled conditions are leveraged to mitigate the impact of confounding variables.
Sources of IGRA variability3,4 | T-SPOT.TB | Other IGRAs |
---|---|---|
Specialized tubes | No | Yes |
On-site refrigeration | No | Yes |
On-site incubation | No | Yes |
Shaking of blood tubes | No | Yes |
Blood volume variability | No | Yes |
The only commercially available IGRA appropriate to be submitted under CPT® code 86481
CPT Code | 86481 |
---|---|
Applicable test | The T-SPOT.TB test |
Description | Tuberculosis test, cell mediated immunity antigen response measurement; enumeration of gamma interferon-producing T-cells in cell suspension |